|
KMID : 1132720200180040037
|
|
Genomics & Informatics
2020 Volume.18 No. 4 p.37 ~ p.37
|
|
|
Transcriptome analysis of iBET-151, a BET inhibitor alone and in combination with paclitaxel in gastric cancer cells
|
|
Kang Sun-Kyoung
Bae Hyun-Joo
Kwon Woo-Sun
Che Jingmin
Kim Tae-Soo
Chung Hyun-Cheol
Rha Sun-Young
|
|
|
Abstract
|
|
|
|
BET inhibitor, as an epigenetic regulator inhibitor, reduces the expression of oncogenes such as Myc and Bcl-2, which affects cancer growth and development. However, it has modest activity because of the narrow therapeutic index. Therefore, combination therapy is necessary to increase the anti-tumor effect. Paclitaxel, an anti-mitotic inhibitor, is used as second-line therapy for gastric cancer (GC) as a monotherapy or combination. In this study, we performed RNA sequencing of GC cells treated with iBET-151 and/or paclitaxel to identify the differentially expressed genes associated with possible mechanisms of synergistic effect. We also performed Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses to determine the most enriched terms and pathways of upregulated and downregulated genes. We found 460 genes in which iBET-151 and paclitaxel combination treatment changed more than single-treatment or no-treatment. Thus, additional functional studies are needed, but our results provide the first evidence of the synergistic effect between iBET-151 and paclitaxel in regulating the transcriptome of GC cells.
|
|
|
KEYWORD
|
|
|
BET inhibitor, combination, differentially expressed genes, gastric cancer, paclitaxel, transcriptome
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|